Protein Summary

TrmH from Escherichia coli

Full name: tRNA (guanosine(18)-2′-O)-methyltransferase
Synonym: SpoU
GI: 84029292
Orf: z5077, ECs4526, SF3691, S4078, b3651
COG: COG0566
UniProt: P0AGJ2
Complex:
Enzyme type: methyltransferase
Position of modification - modification: t:18 - Gm

Comments:

AdoMet is the methyl group donor. SPOUT superfamily.

Protein sequence:

MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHA
VWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSD
NAVDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNV
SVASALILYEAQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAK
RKGLPYPHVNQQGEIEADADWWATMQAAG

Enzymatic activities

Reaction Substrate Type Position
G:Gm tRNA (t) Gln/UUG/prokaryotic cytosol 18
G:Gm tRNA (t) Gln/CUG/prokaryotic cytosol 18
G:Gm tRNA (t) Ile/CAU/prokaryotic cytosol 18
G:Gm tRNA (t) Leu/CAG/prokaryotic cytosol 18
G:Gm tRNA (t) Leu/GAG/prokaryotic cytosol 18
G:Gm tRNA (t) Leu/UAA/prokaryotic cytosol 18
G:Gm tRNA (t) Met/CAU/prokaryotic cytosol 18
G:Gm tRNA (t) Ser/UGA/prokaryotic cytosol 18
G:Gm tRNA (t) Ser/CGA/prokaryotic cytosol 18
G:Gm tRNA (t) Ser/GGA/prokaryotic cytosol 18
G:Gm tRNA (t) Tyr/GUA/prokaryotic cytosol 18

Publications

Title Authors Journal Details PubMed Id DOI
The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity. Persson BC, Jager G, Gustafsson C Nucleic Acids Res [details] 9321663 -
The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and non-substrate tRNA via an induced-fit process. Ochi A, Makabe K, Yamagami R, Hirata A, Sakaguchi R, Hou YM, Watanabe K, Nureki O, Kuwajima K, Hori H... J Biol Chem [details] 23867454 -

Last modification of this entry: 2013-08-05 13:33:23.448824
Edited by a user: magda
Edited content: Changed papers.