Protein Summary

Rra1 from Saccharomyces cerevisiae

Full name: Ribosomal RNA cytidine Acetyltransferase 1
Synonym: KRE33
GI: 6324197
Orf: N1216, N1858
COG: COG1444
UniProt: P53914
Structures: | |
Complex:
Enzyme type: acetyltransferase
Position of modification - modification: t:12 - ac4C
s:1773(1510) - ac4C
s:1280(1060) - ac4C

Comments:

RRA1 (KRE33) is a cytosine acetyltransferase with specificity towards both 18S rRNA and serine and leucine tRNAs. KRE33 is required for formation of ac4C1280 (helix 34) and ac4C1773 (helix 45) on 18S rRNA. KRE33 is required for pre-rRNA processing steps leading to 18S rRNA synthesis but 18S rRNA acetylation is not required for pre-rRNA processing. KRE33 is additionally required for ac4C12 on serine and leucine tRNAs. KRE33 interacts in vivo with TAN1. TAN1 is also required for tRNA acetylation but does not carry any acetyltransferase domain. On this basis, TAN1 was suggested to act as a tRNA adaptor to KRE33. NAT10 is the human homolog of KRE33 and THUMPD1 the human homolog of TAN1.

Protein sequence:

MAKKAIDSRIPSLIRNGVQTKQRSIFVIVGDRARNQLPNLHYLMMSADLK
MNKSVLWAYKKKLLGFTSHRKKRENKIKKEIKRGTREVNEMDPFESFISN
QNIRYVYYKESEKILGNTYGMCILQDFEALTPNLLARTIETVEGGGIVVI
LLKSMSSLKQLYTMTMDVHARYRTEAHGDVVARFNERFILSLGSNPNCLV
VDDELNVLPLSGAKNVKPLPPKEDDELPPKQLELQELKESLEDVQPAGSL
VSLSKTVNQAHAILSFIDAISEKTLNFTVALTAGRGRGKSAALGISIAAA
VSHGYSNIFVTSPSPENLKTLFEFIFKGFDALGYQEHIDYDIIQSTNPDF
NKAIVRVDIKRDHRQTIQYIVPQDHQVLGQAELVVIDEAAAIPLPIVKNL
LGPYLVFMASTINGYEGTGRSLSLKLIQQLRNQNNTSGRESTQTAVVSRD
NKEKDSHLHSQSRQLREISLDEPIRYAPGDPIEKWLNKLLCLDVTLIKNP
RFATRGTPHPSQCNLFVVNRDTLFSYHPVSENFLEKMMALYVSSHYKNSP
NDLQLMSDAPAHKLFVLLPPIDPKDGGRIPDPLCVIQIALEGEISKESVR
NSLSRGQRAGGDLIPWLISQQFQDEEFASLSGARIVRIATNPEYASMGYG
SRAIELLRDYFEGKFTDMSEDVRPKDYSIKRVSDKELAKTNLLKDDVKLR
DAKTLPPLLLKLSEQPPHYLHYLGVSYGLTQSLHKFWKNNSFVPVYLRQT
ANDLTGEHTCVMLNVLEGRESNWLVEFAKDFRKRFLSLLSYDFHKFTAVQ
ALSVIESSKKAQDLSDDEKHDNKELTRTHLDDIFSPFDLKRLDSYSNNLL
DYHVIGDMIPMLALLYFGDKMGDSVKLSSVQSAILLAIGLQRKNIDTIAK
ELNLPSNQTIAMFAKIMRKMSQYFRQLLSQSIEETLPNIKDDAIAEMDGE
EIKNYNAAEALDQMEEDLEEAGSEAVQAMREKQKELINSLNLDKYAINDN
SEEWAESQKSLEIAAKAKGVVSLKTGKKRTTEKAEDIYRQEMKAMKKPRK
SKKAAN

Enzymatic activities

Reaction Substrate Type Position
C:ac4C rRNA (r) SSU/18S/cytosolic 1773
C:ac4C rRNA (r) SSU/18S/cytosolic 1280
C:ac4C tRNA (t) Leu/UAG/cytosolic 12
C:ac4C tRNA (t) Leu/CAA/cytosolic 12
C:ac4C tRNA (t) Leu/UAA/cytosolic 12
C:ac4C tRNA (t) Ser/CGA/cytosolic 12
C:ac4C tRNA (t) Ser/UGA/cytosolic 12
C:ac4C tRNA (t) Ser/IGA/cytosolic 12
C:ac4C tRNA (t) Ser/GCU/cytosolic 12

Publications

Title Authors Journal Details PubMed Id DOI
A single acetylation of 18S rRNA is essential for biogenesis of the small ribosomal subunit in Saccharomyces cerevisiae. Ito S, Akamatsu Y, Noma A, Kimura S, Miyauchi K, Ikeuchi Y, Suzuki T, Suzuki T... J Biol Chem [details] 25086048 -
Yeast Kre33 and human NAT10 are conserved 18S rRNA cytosine acetyltransferases that modify tRNAs assisted by the adaptor Tan1/THUMPD1. Sharma S, Langhendries JL, Watzinger P, Kotter P, Entian KD, Lafontaine DL... Nucleic Acids Res [details] 25653167 -

Links

_PubMed_

Last modification of this entry: 2015-02-18 09:42:34.502152
Edited by a user: magda
Edited content: Changed comments.