Protein Summary

Trm4 from Homo sapiens

Full name: tRNA (cytosine(34)-C(5))-methyltransferase
Synonym: hMisu, SAKI
GI: 39995082
Orf: NSUN2
COG: COG0144
UniProt: Q08J23
Structures: | |
Complex:
Enzyme type: methyltransferase
Position of modification - modification: pre-tRNA:48 - m5C
pre-tRNA:34 - m5C
m:many - m5C
other:many - m5C
t:48 - m5C
t:49 - m5C
t:50 - m5C

Comments:

Multisite specific enzyme. Orthologue of yeast Trm4. It modifies not only tRNA but also RPPH1 subunit of RNase P and some mRNAs. hTrm4 partially complements the lack of the endogenous SceTrm4p. Exists in two isoforms.

Protein sequence:

MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEH
YYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFK
ELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLV
SETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHA
DMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQ
IDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQL
RIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSN
ELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEK
LQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETR
ESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDG
VCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLT
RTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCA
FRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAK
DLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLR
MMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEAN
SPDVTAGCDPAGVHPPR

Enzymatic activities

Reaction Substrate Type Position
C:m5C pre-tRNA (pre-t) Leu/CAA/prokaryotic cytosol 34
C:m5C mRNA (m)
C:m5C other (o)
C:m5C tRNA (t) Leu/CAA/prokaryotic cytosol 48
C:m5C pre-tRNA (pre-t) Leu/CAA/prokaryotic cytosol 48
C:m5C tRNA (t) Gly/GCC/cytosolic 48
C:m5C tRNA (t) Gly/GCC/cytosolic 49
C:m5C tRNA (t) Gly/GCC/unknown 50

Publications

Title Authors Journal Details PubMed Id DOI
Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu (CAA). Brzezicha B, Schmidt M, Makalowska I, Jarmolowski A, Pienkowska J, Szweykowska-Kulinska Z... Nucleic Acids Res [details] 17071714 -
Widespread occurrence of 5-methylcytosine in human coding and non-coding RNA. Squires JE, Patel HR, Nousch M, Sibbritt T, Humphreys DT, Parker BJ, Suter CM, Preiss T... Nucleic Acids Res [details] 22344696 -
Function and detection of 5-methylcytosine in eukaryotic RNA. Squires JE, Preiss T... Epigenomics [details] 22122054 -
The Human tRNA m ( 5) C methyltransferase Misu is multisite-specific. Auxilien S, Guerineau V, Szweykowska-Kulinska Z, Golinelli-Pimpaneau B... RNA Biol [details] 22995836 -
Methylation by NSun2 represses the levels and function of microRNA 125b. Yuan S, Tang H, Xing J, Fan X, Cai X, Li Q, Han P, Luo Y, Zhang Z, Jiang B, Dou Y, Gorospe M, Wang W... Mol Cell Biol [details] 25047833 -

Links

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Last modification of this entry: 2014-11-21 15:14:41.076712
Edited by a user: magda
Edited content: Changed papers.