Protein Summary

TrmI from Mycobacterium tuberculosis

Full name: tRNA (adenine(58)-N(1))-methyltransferase TrmI
Synonym: Rv2118c, MT2178
GI: 81669088
Orf:
COG: COG2519
UniProt: O33253
Structures: | 1I9G |
Complex:
Enzyme type: methyltransferase
Position of modification - modification: t:58 - m1A

Comments:

Homologous to yeast Gcd14 (Trm61) and T. thermophilus TmrI. S-adenosyl-L-methionine is the methyl group donor. Homotetramer.

Protein sequence:

MSATGPFSIGERVQLTDAKGRRYTMSLTPGAEFHTHRGSIAHDAVIGLEQ
GSVVKSSNGALFLVLRPLLVDYVMSMPRGPQVIYPKDAAQIVHEGDIFPG
ARVLEAGAGSGALTLSLLRAVGPAGQVISYEQRADHAEHARRNVSGCYGQ
PPDNWRLVVSDLADSELPDGSVDRAVLDMLAPWEVLDAVSRLLVAGGVLM
VYVATVTQLSRIVEALRAKQCWTEPRAWETLQRGWNVVGLAVRPQHSMRG
HTAFLVATRRLAPGAVAPAPLGRKREGRDG

Enzymatic activities

Reaction Substrate Type Position
A:m1A tRNA (t) many/many/prokaryotic cytosol 58

Publications

Title Authors Journal Details PubMed Id DOI
Structural comparison of tRNA m(1)A58 methyltransferases revealed different molecular strategies to maintain their oligomeric architecture under extreme conditions. Guelorget A, Barraud P, Tisne C, Golinelli-Pimpaneau B BMC Struct Biol [details] 22168821 -
Mycobacterium tuberculosis Rv2118c codes for a single-component homotetrameric m1A58 tRNA methyltransferase. Varshney U, Ramesh V, Madabushi A, Gaur R, Subramanya HS, RajBhandary UL Nucleic Acids Res [details] 14960715 -
Crystal structure of Rv2118c: an AdoMet-dependent methyltransferase from Mycobacterium tuberculosis H37Rv. Gupta A, Kumar PH, Dineshkumar TK, Varshney U, Subramanya HS J Mol Biol [details] 11554794 -

Last modification of this entry: Feb. 4, 2014