Protein Summary

Nop2 from Saccharomyces cerevisiae

Full name: Nucleolar protein 2
Synonym: Yna1, N2428, YNL2428W
GI: 730166
Orf: YNL061W
COG: COG0144
UniProt: P40991
Complex:
Enzyme type: methyltransferase
Position of modification - modification: l:2870(2501) - m5C

Comments:

The nucleolar Nop2 protein binds 27S pre-rRNA. Loss of m5C2870 affects ribosome synthesis and processing.

Protein sequence:

MGSRRHKNKQAAPPTLEEFQARKEKKANRKLEKGKRPSTTQGDEVSDRKK
KKSKPFKKSRKEEEEVVEEDKDLPEVDLEELSKARKSLFDDEEDDDEAGL
VDEELKDEFDLEQEYDYDEDEDNDAHPIFSDDDDEADLEELNAQNMEALS
KKLDEEEAEEAEEAEMELVEAENMQPRADILPTEEQEEMMAQETPNLTST
RTRMIEIVKVLENFKTLGAEGRSRGEYVDRLLKDICEYFGYTPFLAEKLF
NLFSPAEAMEFFEANEIARPITIRTNTLKTRRRDLAQTLVNRGVNLQPIG
SWTKVGLQIFDSQVPIGATPEYLAGHYILQAASSFLPVIALDPHENERIL
DMAAAPGGKTTYISAMMKNTGCVFANDANKSRTKSLIANIHRLGCTNTIV
CNYDAREFPKVIGGFDRILLDAPCSGTGVIGKDQSVKVSRTEKDFIQIPH
LQKQLLLSAIDSVDCNSKHGGVIVYSTCSVAVEEDEAVIDYALRKRPNVK
LVDTGLAIGKEAFTSYRGKKFHPSVKLARRYYPHTYNVDGFFVAKFQKIG
PSSFDDNQASAKEKETAARKEALEEGIIHSDFATFEDEEDDKYIEKSVKN
NLLKKGVNPKAKRPSNEK

Enzymatic activities

Reaction Substrate Type Position
C:m5C rRNA (r) LSU-L/25S/cytosolic 2870

Publications

Title Authors Journal Details PubMed Id DOI
Yeast NOP2 encodes an essential nucleolar protein with homology to a human proliferation marker. de Beus E, Brockenbrough JS, Hong B, Aris JP J Cell Biol [details] 7806561 -
Nop2p is required for pre-rRNA processing and 60S ribosome subunit synthesis in yeast. Hong B, Brockenbrough JS, Wu P, Aris JP Mol Cell Biol [details] 8972218 -
Temperature sensitive nop2 alleles defective in synthesis of 25S rRNA and large ribosomal subunits in Saccharomyces cerevisiae. Hong B, Wu K, Brockenbrough JS, Wu P, Aris JP Nucleic Acids Res [details] 11452018 -
Phylogenetic analysis of the eukaryotic RNA (cytosine-5)-methyltransferases. Pavlopoulou A, Kossida S Genomics [details] 19135144 -
RNA methyltransferases utilize two cysteine residues in the formation of 5-methylcytosine. King MY, Redman KL Biochemistry [details] 12220187 -
Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively. Sharma S, Yang J, Watzinger P, Kotter P, Entian KD... Nucleic Acids Res [details] 23913415 -
The methyltransferase adaptor protein Trm112 is involved in biogenesis of both ribosomal subunits. Sardana R, Johnson AW... Mol Biol Cell [details] 22956767 -

Last modification of this entry: 2017-09-14 17:12:08.104028
Edited by a user: elapurta
Edited content: No fields changed.