Protein Summary

MnmE from Thermotoga maritima

Full name: tRNA modification GTPase MnmE
Synonym: TrmE
GI: 15643037
Orf:
COG: COG0486
UniProt: Q9WYA4
Structures: | 1XZP | 1XZQ |
Complex: MnmE/MnmG
Enzyme type: GTPase
Position of modification - modification: t:34 - cmnm5s2U
t:34 - cmnm5U

Comments:

Homologue of E. coli MnmE. Hydrolyses GTP. MnmE works in complex with MnmG (GidA). Catalyzes two independent reactions according to substrate (glycine or ammonium).

Protein sequence:

MDTIVAVATPPGKGAIAILRLSGPDSWKIVQKHLRTRSKIVPRKAIHGWI
HENGEDVDEVVVVFYKSPKSYTGEDMVEVMCHGGPLVVKKLLDLFLKSGA
RMAEPGEFTKRAFLNGKMDLTSAEAVRDLIEAKSETSLKLSLRNLKGGLR
DFVDSLRRELIEVLAEIRVELDYPDEIETNTGEVVTRLERIKEKLTEELK
KADAGILLNRGLRMVIVGKPNVGKSTLLNRLLNEDRAIVTDIPGTTRDVI
SEEIVIRGILFRIVDTAGVRSETNDLVERLGIERTLQEIEKADIVLFVLD
ASSPLDEEDRKILERIKNKRYLVVINKVDVVEKINEEEIKNKLGTDRHMV
KISALKGEGLEKLEESIYRETQEIFERGSDSLITNLRQKQLLENVKGHLE
DAIKSLKEGMPVDMASIDLERALNLLDEVTGRSFREDLLDTIFSNFCVGK

Enzymatic activities

Reaction Substrate Type Position
Um:cmnm5Um tRNA (t)   34
s2U:nm5s2U tRNA (t)   34
se2U:nm5se2U tRNA (t)   34
s2U:cmnm5s2U tRNA (t)   34
se2U:cmnm5se2U tRNA (t)   34
U:nm5U tRNA (t)   34
U:cmnm5U tRNA (t)   34

Publications

Title Authors Journal Details PubMed Id DOI
The structure of the TrmE GTP-binding protein and its implications for tRNA modification. Scrima A, Vetter IR, Armengod ME, Wittinghofer A EMBO J [details] 15616586 -
Characterization of GTPase activity of TrmE, a member of a novel GTPase superfamily, from Thermotoga maritima. Yamanaka K, Hwang J, Inouye M J Bacteriol [details] 11092873 -
Enzymology of tRNA modification in the bacterial MnmEG pathway. Armengod ME, Moukadiri I, Prado S, Ruiz-Partida R, Benitez-Paez A, Villarroya M, Lomas R, Garzon MJ, Martinez-Zamora A, Meseguer S, Navarro-Gonzalez C Biochimie [details] 22386868 -

Links

_PubMed_

Last modification of this entry: 2012-07-03 17:16:00.971308
Edited by a user: magda
Edited content: Changed protein_sequence, if_enzyme, nomenclature, catalytic_domain_fold and comments.