Protein Summary

Tgt from Escherichia coli

Full name: tRNA guanosine(34) transglycosylase
Synonym: JW0396
GI: 67473024
Orf: b0406
COG: COG0343
UniProt: P0A847
Complex:
Enzyme type: transglycosylase
Position of modification - modification: t:34 - QtRNA

Comments:

Reaction preQ0(b):preQ0 do not occur under physiological conditions, because normally all preQ0(b) is converted to preQ1(b) by QueF. Do not exist in S. cerevisiae, exist in other yeast (Tgt, O94460, GI20514973, SPAC1687.19c). Structure in complex with tRNA was solved for Tgt enzyme from Zymomonas mobilis (1Q2R).

Protein sequence:

MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATG
AQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGD
IRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPA
DWDYAKRSMEMSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVK
GLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKPRYLMGVGKPE
DLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSDTGPLDP
ECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIE
EGKLESFVTDFYQRQGREVPPLNVD

Enzymatic activities

Reaction Substrate Type Position
preQ1base:preQ1tRNA tRNA (t)   34

Publications

Title Authors Journal Details PubMed Id DOI
New function of vitamin B12: cobamide-dependent reduction of epoxyqueuosine to queuosine in tRNAs of Escherichia coli and Salmonella typhimurium. Frey B, McCloskey J, Kersten W, Kersten H J Bacteriol [details] 3129401 -

Links

_EcoCyc_

Last modification of this entry: 2012-07-04 13:16:06.957709
Edited by a user: magda
Edited content: Changed protein_sequence, if_enzyme, nomenclature, catalytic_domain_fold and comments.