Protein Summary

Man/Gal-Q-transferase from various eukaryotes

Full name: tRNA-queuine glycosyltransferase
Synonym:
GI: None
Orf:
COG:
UniProt:
Complex:
Enzyme type: hexose transferase
Position of modification - modification: t:34 - galQtRNA
t:34 - manQtRNA

Comments:

Hexose-transferase (Htr). Utilizes GDP-mannose, GDP-galactose In tRNAAsp (anticodon QUC) and tRNATyr (anticodon QYA) from certain eukaryotic cells (e.g. mammalian hepatocytes), the nucleoside Q-34 is further hypermodified into a glycosylated derivative by tRNA-queuine glycosyltransferase. This enzymatic activity was detected in rat liver extract and Xenopus leavis oocytes but the protein was not purified.

Enzymatic activities

Reaction Substrate Type Position
QtRNA:galQtRNA tRNA (t) Tyr/GUA/prokaryotic cytosol 34
QtRNA:manQtRNA tRNA (t) Asp/GUC/prokaryotic cytosol 34

Publications

Title Authors Journal Details PubMed Id DOI
The RNA Modification Database: 1999 update. Rozenski J, Crain PF, McCloskey JA Nucleic Acids Res [details] 9847178 -
Enzymatic formation of queuosine and of glycosyl queuosine in yeast tRNAs microinjected into Xenopus laevis oocytes. The effect of the anticodon loop sequence. Haumont E, Droogmans L, Grosjean H Eur J Biochem [details] 3117541 -
Structure, biosynthesis, and function of queuosine in transfer RNA. Nishimura S Prog Nucleic Acid Res Mol Biol [details] 6410456 -
Enzymatic synthesis of Q nucleoside containing mannose in the anticodon of tRNA: isolation of a novel mannosyltransferase from a cell-free extract of rat liver. Okada N, Nishimura S Nucleic Acids Res [details] 20603 -

Last modification of this entry: 2012-07-03 17:10:22.589858
Edited by a user: magda
Edited content: Changed if_enzyme, nomenclature and comments.