"2'-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA."

Huber SM, van Delft P, Tanpure A, Miska EA, Balasubramanian S...

Published 2017-01-08 in J Am Chem Soc volume 139 .

Pubmed ID: 28107630
DOI identifier: -

5-Hydroxymethylcytidine (hm(5)C) was recently identified as a direct metabolite of m(5)C in RNA. We investigated the stability of hm(5)C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m(5)C in RNA, namely 2'-O-methyl-5-hydroxymethylcytidine (hm(5)Cm). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this new RNA modification.

Last modification of this entry: April 1, 2019