"The use of labilebaseprotectinggroups in oligoribonucleotidesynthesis"

Carole Chaix, Didier Molko, Robert Téoule

Published 1988-09-29 in Tetrahedron Letters volume Volume 30, Issue 1, 1989 .

Pubmed ID: -
DOI identifier: 10.1016/S0040-4039(01)80326-9

The use of phenoxyacetyl group for the protection of the exocyclic amino function of purine bases and acetyl group for cytosine in oligonucleotide synthesis by the cyanoethylphosphoramidite approach is described. A side reaction - i.e. partial replacement of phenoxyacetyl group of protected guanines by acetyl group - was observed during the capping step. It can be avoided by the use of phenoxyacetic anhydride in place of acetic anhydride.

Entry added on: 2012-06-27 08:52:26.341265, by a user: mark