"Crystallization and preliminary X-ray characterization of queD from Bacillus subtilis, an enzyme involved in queuosine biosynthesis."

Cicmil N, Shi L



Published 2008-01-01 in Acta Crystallogr Sect F Struct Biol Cryst Commun volume 64 .

Pubmed ID: 18259064
DOI identifier: -

Abstract:
QueD (previously named ykvK) is one of several enzymes involved in the biosynthesis of the hypermodified nucleoside queuosine. Queuosine is incorporated into tRNA at position 34 of four tRNAs: tRNA(His), tRNA(Asp), tRNA(Asn) and tRNA(Tyr). The crystallization and preliminary X-ray crystallographic studies of queD are described here. The recombinant protein from Bacillus subtilis was overproduced in Escherichia coli and crystallized using the hanging-drop vapor-diffusion method from 25% PEG 600, 100 mM NaCl and sodium citrate buffer pH 5.5 at 291 K. The crystals diffract to 3.6 A resolution and belong to the cubic space group F4(1)32, with unit-cell parameter a = 240.88 A.


Last modification of this entry: Sept. 6, 2012