"Novel methyltransferase for modified uridine residues at the wobble position of tRNA."

Kalhor HR, Clarke S



Published 2003-12-01 in Mol Cell Biol volume 23 .

Pubmed ID: 14645538
DOI identifier: -

Abstract:
We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of the YML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu). In intact yeast cells, disruption of the TRM9 gene results in the complete loss of these modified wobble bases and increased sensitivity at 37 degrees C to paromomycin, a translational inhibitor. These results suggest a role for this potentially reversible methyl esterification reaction when cells are under stress.


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Last modification of this entry: Sept. 6, 2012