"Site-directed cleavage of RNA."

Shibahara S, Mukai S, Nishihara T, Inoue H, Ohtsuka E, Morisawa H



Published 1987-06-11 in Nucleic Acids Res volume 15 .

Pubmed ID: 2438655
DOI identifier: -

Abstract:
Using complementary chimeric oligonucleotides containing deoxyribonucleotides and 2'-O-methylribonucleotides (1), enzymatically synthesized RNA (90 mer) were cleaved at a single site with Escherichia coli RNaseH, either at a hairpin loop or at a stem region. Especially, site-specific cleavage occurred in even the target region being enclosed within a stable, base-paired stem. The method is proved to be generally applicable to RNA containing secondary structures.


Last modification of this entry: Sept. 6, 2012