"The synthesis of 2'-O-[(Triisopropylsilyl)oxy] methyl (TOM) phosphoramidites of methylated ribonucleosides (m1G, m2G, m22G, m1I, m3U, m4C, m6A, m62A) for use in automated RNA solid-phase synthesis"

HĂ–BARTNER Claudia ; KREUTZ Christoph ; FLECKER Elke ; OTTENSCHLĂ„GER Elke ; PILS Werner ; GRUBMAYR Karl ; MICURA Ronald ;



Published 2003-05-01 in Monatshefte fuer Chemie .

Pubmed ID: -
DOI identifier: -

Abstract:
The straightforward synthesis of eight methylated ribonucleoside phosphoramidites is described. These building blocks allow for incorporation of the naturally occuring nucleosides 1-methylguanosine (m1G), N2-metylguanosine (m2G), N2,N2-dimethylguanosine (m22G), 1-methylinosine (m1I), 3-methyluridine (m3U), N4-methylcytidine (m4C), N6-methyladenosine (m6A), and N6,N6-dimethyladenosine (m62A) into oligoribonucleotides by automated RNA solid-phase synthesis. In all cases, the ribose 2'-hydroxyl group of the building blocks is masked by the recently introduced [(triisopropylsilyl)oxy[mehtyl (TOM) group.


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