"Structure of wye (Yt base) and wyosine (Yt) from Torulopsis utilis phenylalanine transfer ribonucleic acid."

Kasai H, Goto M, Ikeda K, Zama M, Mizuno Y, Takemura S, Matsuura S, Sugimoto T, Goto T

Published 1976-01-24 in Biochemistry volume 15 .

Pubmed ID: 942858
DOI identifier: -

A fluorescent base wye (Yt base) was isolated from Torulopsis utilis tRNAPhe. The structure was established as 4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazo[1,2-alpha]purine based on ultraviolet (uv), nuclear magnetic resonance (NMR), and mass spectra, and by direct comparison with synthetic material. The nucleoside, wyosine (Yt), was isolated from purified tRNAPhe by enzymatic degradations followed by column and thin-layer chromatographies. The structure of wyosine is proposed as 3-ribofuranosyl-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazol[1,2-alpha]purin e (the ribosyl group is attached to the N-9 position of guanine nucleus) on the basis of comparison with model compounds.

Last modification of this entry: Sept. 6, 2012