"Identification of a gene involved in the generation of 4-thiouridine in tRNA."

Mueller EG, Buck CJ, Palenchar PM, Barnhart LE, Paulson JL



Published 1998-06-01 in Nucleic Acids Res volume 26 .

Pubmed ID: 9592144
DOI identifier: -

Abstract:
All organisms modify the bases of their RNA after transcription. Relatively little is known about the functions that these chemical alterations serve and, with very few exceptions, even less has been established regarding the enzymology involved. One modified base of known function is 4-thiouridine at position 8 of certain bacterial tRNAs, which serves as a photosensor for near-UV light. A gene involved in the conversion of uridine at position 8 into 4-thiouridine has been identified by genetic screening and its role in 4-thiouridine generation has been confirmed biochemically. This same gene, thiI , has recently been shown to play a role in thiamin biosynthesis. The purification and characteristics of the purified protein are also reported.


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Last modification of this entry: Sept. 6, 2012