Modification Summary

N6-threonylcarbamoyladenosine

[show modification pathway]
t6A

Summary

Full nameN6-threonylcarbamoyladenosine
Short namet6A
New Nomenclature62A
RNAMods abbreviation6
HTML abbreviation6
Enzymes Sua5 (Saccharomyces cerevisiae)
TsaD (Escherichia coli)
Found in phylogenyEubacteria, Eukaryota
Found in RNAtRNA
SMILES codeCC(O)C(N)C(=O)NC(=O)Nc3ncnc2c3ncn2[C@@H]1O[C@H](CO)[C@H](O)C1O

LC-MS Information

Sum formulaC15O8N6H20
Monoisotopic mass412.134261
Average mass412.359848
HRMS mass412.133712
Normalized LC elution time *1,62 (Kellner 2014)
LC elution order/characteristicsbetween A and m6A (Kellner 2014)
Mass transition-132 (Kellner 2014, Su 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -
Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry. Su D, Chan CT, Gu C, Lim KS, Chionh YH, McBee ME, Russell BS, Babu IR, Begley TJ, Dedon PC... Nat Protoc [details] 24625781 -

Download [.mol file for t6A]

Chemical groups contained

aminoacyl groupthreonyl
othercarbamoyl

Reactions producing N6-threonylcarbamoyladenosine

Name
A:t6A
ct6A:t6A

Reactions starting from N6-threonylcarbamoyladenosine

Name
t6A:m6t6A
t6A:ms2t6A
t6A:ct6A

Occurrence in PDB structures

PDB-ID chain residue
1feq A T6A /37
1fl8 A T6A /37
1xmo X T6A /37
1xmq X T6A /37
1yfg T6A /37
2g1g A T6A /11

Last modification of this entry: 2017-07-05 16:19:18.448940
Edited by a user: pboccaletto
Edited content: Changed lc_elution_time, lc_elution_comment, lc_reference and mass_trans.