Modification Summary
2-thiouridine
[show modification pathway]
Summary
| Full name | 2-thiouridine |
| Short name | s2U |
| New Nomenclature | 2U |
| RNAMods abbreviation | 2 |
| HTML abbreviation | 2 |
| Enzymes |
MnmA (Escherichia coli) Mtu1 (Saccharomyces cerevisiae) Ncs2 (Saccharomyces cerevisiae) Ncs6 (Saccharomyces cerevisiae) TrmU (Homo sapiens) |
| Found in phylogeny | Eubacteria, Eukaryota |
| Found in RNA | tRNA |
| PubChem ID | 3036443 |
| SMILES code | C3(OC(CO)C(O)C(O)3)N1C=CC(=O)NC1(=S) |
LC-MS Information
| Sum formula | C9O5N2H12S1 |
| Monoisotopic mass | 260.0467 |
| Average mass | 260.2694 |
| [M+H]+ | 261.0545 |
| Product ions | 129 |
| Normalized LC elution time * | 1,06 (Kellner 2014) |
| LC elution order/characteristics | between G and A (Kellner 2014) |
* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.
LC-MS Publications
| Title | Authors | Journal | Details | ||
|---|---|---|---|---|---|
| Absolute and relative quantification of RNA modifications via biosynthetic isotopomers. | Kellner S, Ochel A, Thuring K, Spenkuch F, Neumann J, Sharma S, Entian KD, Schneider D, Helm M... | Nucleic Acids Res | [details] | 25129236 | - |
Download [.mol file for s2U]
Chemical groups contained
| heavy atom | sulfur |
Reactions producing 2-thiouridine
| Name |
|---|
| U:s2U |
Reactions starting from 2-thiouridine
| Name |
|---|
| s2U:se2U |
| s2U:nm5s2U |
| s2U:cmnm5s2U |
| s2U:tm5s2U |
| s2U:s2Um |
| s2U:ges2U |
Occurrence in PDB structures
| PDB-ID | chain | residue |
|---|---|---|
| 2c4y | R | SUR /11 |
| 2c4y | S | SUR /11 |
| 2c4z | R | SUR /10 |
| 2c4z | R | SUR /11 |
| 2c4z | S | SUR /10 |
| 2c4z | S | SUR /11 |
| 2k7e | B | SUR/3 |
Last modification of this entry: 2017-07-05 17:05:24.500293
Edited by a user: pboccaletto
Edited content: Changed lc_elution_time, lc_elution_comment, lc_reference and mass_trans.
