Modification Summary

5-carbamoylmethyluridine

[show modification pathway]
ncm5U

Summary

Full name5-carbamoylmethyluridine
Short namencm5U
New Nomenclature53U
RNAMods abbreviation&
HTML abbreviation&
Enzymes
Found in phylogenyEukaryota
Found in RNAtRNA
SMILES codeC3(OC(CO)C(O)C(O)3)N1C=C(CC(=O)N)C(=O)NC1(=O)

LC-MS Information

Sum formulaC11O7N3H15
Monoisotopic mass301.091
Average mass301.2563
[M+H]+302.0988
Product ions170/153/125
Normalized LC elution time *0,50 (Kellner 2014)
LC elution order/characteristicsbetween C and U (Kellner 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -
Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry. Su D, Chan CT, Gu C, Lim KS, Chionh YH, McBee ME, Russell BS, Babu IR, Begley TJ, Dedon PC... Nat Protoc [details] 24625781 -

Download [.mol file for ncm5U]

Chemical groups contained

methyl groupmethyl at aromatic C
othercarbamoyl

Reactions producing 5-carbamoylmethyluridine

Name
U:ncm5U

Reactions starting from 5-carbamoylmethyluridine

Name
ncm5U:ncm5Um
ncm5U:nchm5U
ncm5U:ncm5s2U

Last modification of this entry: 2017-10-06 16:24:08.974324
Edited by a user: pboccaletto
Edited content: Changed mass_prot.