Modification Summary


[show modification pathway]


Full name5-methoxyuridine
Short namemo5U
New Nomenclature501U
RNAMods abbreviation5
HTML abbreviation5
Enzymes CmoB (Escherichia coli)
Found in phylogenyEubacteria
Found in RNAtRNA
PubChem ID169704
SMILES codeC3(OC(CO)C(O)C(O)3)N1C=C(OC)C(=O)NC1(=O)

LC-MS Information

Sum formulaC10O7N2H14
Monoisotopic mass274.0801
Average mass274.2306
Product ions143
Normalized LC elution time * not available
LC elution order/characteristics not available

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

Download [.mol file for mo5U]


mo5U is a modification characteristic for gram positive bacteria (B. subtilis, Firmicutes). Gram negative bacteria have cmo5U and mcmo5U in this position (E. coli, Protebacteria). Null mutation of cmoA in S. enterica results in the accummulation of tRNA containing mo5U34 and ho5U34.

Chemical groups contained


Reactions producing 5-methoxyuridine


Reactions starting from 5-methoxyuridine



Title Authors Journal Details PubMed Id DOI
5-methoxyuridine: a new minor constituent located in the first position of the anticodon of tRNAAla, tRNAThr, and tRNAVal from Bacillus subtilis. Murao K, Hasegawa T, Ishikura H Nucleic Acids Res [details] 825836 -
The modified wobble nucleoside uridine-5-oxyacetic acid in tRNAPro(cmo5UGG) promotes reading of all four proline codons in vivo. Nasvall SJ, Chen P, Bjork GR RNA [details] 15383682 -

Last modification of this entry: 2013-05-10 16:36:58.993978
Edited by a user: magda
Edited content: Kingdom/s added to a modification