Modification Summary
uridine 5-oxyacetic acid methyl ester
[show modification pathway]
Summary
Full name | uridine 5-oxyacetic acid methyl ester |
Short name | mcmo5U |
New Nomenclature | 503U |
RNAMods abbreviation | υ |
HTML abbreviation | υ |
Enzymes |
|
Found in phylogeny | Eubacteria |
Found in RNA | tRNA |
PubChem ID | 191808 |
SMILES code | C3(OC(CO)C(O)C(O)3)N1C=C(OCC(=O)OC)C(=O)NC1(=O) |
LC-MS Information
Sum formula | C12O9N2H16 |
Monoisotopic mass | 332.0856 |
Average mass | 332.2672 |
[M+H]+ | 333.0934 |
Product ions | 183/201/297/315/129 |
Normalized LC elution time * | 1,47 (Kellner 2014)
|
LC elution order/characteristics | between A and m6A (Kellner 2014) |
* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.
LC-MS Publications
Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
Profiling of RNA modifications by multiplexed stable isotope labelling. |
Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. |
Chem Commun (Camb). |
[details]
|
24567952
|
-
|
Comments
Unstable under usual methods of detection, not easily detectable (Glenn Bjork, personal communication).
cmo5U (and mcmo5U) is a modification characteristic for gram negative bacteria (E. coli, Protebacteria). Gram positive bacteria (B. subtilis, Firmicutes) have mo5U in this position.
Chemical groups contained
other | carboxymethyl |
other | methoxy |
other | methylester |
Reactions producing uridine 5-oxyacetic acid methyl ester
Reactions starting from uridine 5-oxyacetic acid methyl ester
Publications
Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
The identification of the tRNA substrates for the supK tRNA methylase. |
Pope WT, Brown A, Reeves RH |
Nucleic Acids Res |
[details]
|
347399
|
-
|
Last modification of this entry: 2017-07-05 16:18:00.807219
Edited by a user: pboccaletto
Edited content: Changed lc_elution_time, lc_elution_comment, lc_reference and mass_trans.