Modification Summary

8-methyladenosine

[show modification pathway]
m8A

Summary

Full name8-methyladenosine
Short namem8A
New Nomenclature8A
RNAMods abbreviationรข
HTML abbreviationâ
Enzymes Cfr (Staphylococcus sciuri)
Found in phylogenyEubacteria
Found in RNArRNA
SMILES codeCC1=NC2=C(N)N=CN=C2[N]1C3OC(CO)C(O)C3O

LC-MS Information

Sum formulaC11O4N5H15
Monoisotopic mass281.112403
Average mass281.271879
HRMS mass281.111855
Normalized LC elution time
LC elution order/characteristics
LC literature references
MS Data File
MS/MS Data File
MS/MS/MS (base ion) Data File
MS literature references

Download [.mol file for m8A]

Comments

The methyltransferase Cfr making m8A, has only been found in Staphylococcus sciuri so far, but it has been investigated in E. coli by expression of Cfr from a plasmid coded gene. It methylates A2503 in E. coli 23S RNA to m8A.

Also see: Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria. Giessing AM, Jensen SS, Rasmussen A, Hansen LH, Gondela A, Long K, Vester B, Kirpekar F. RNA. 2009 Feb;15(2):327-36.
PMID: 19144912

Chemical groups contained

methyl groupmethyl at aromatic C

Reactions producing 8-methyladenosine

Name
A:m8A

Reactions starting from 8-methyladenosine

Name
m8A:m2,8A

Last modification of this entry: 2013-05-10 16:37:02.455056
Edited by a user: magda
Edited content: Kingdom/s added to a modification