Modification Summary

7-methylguanosine

[show modification pathway]
m7G

Summary

Full name7-methylguanosine
Short namem7G
New Nomenclature7G
RNAMods abbreviation7
HTML abbreviation7
Enzymes Abd1 (Saccharomyces cerevisiae)
ArmA (Escherichia coli)
Bud23 (Saccharomyces cerevisiae)
RlmKL (Escherichia coli)
RmtB (Escherichia coli)
RsmG (Escherichia coli)
RsmG (Bacillus subtilis)
RsmG (Thermus thermophilus)
Sgm (Micromonospora zionensis)
TRMB (Homo sapiens)
Trm8 (Saccharomyces cerevisiae)
TrmB (Escherichia coli)
TrmB (Bacillus subtilis)
Found in phylogenyEubacteria, Eukaryota
Found in RNArRNA, tRNA
SMILES codeNC3=Nc2c([n+](C)cn2[C@H]1O[C@@H](CO)[C@@H](O)C1O)C([O-])N3

LC-MS Information

Sum formulaC11O5N5H17
Monoisotopic mass299.123
Average mass299.2867
[M+H]+298.1151
Product ions166
Normalized LC elution time *0,83 (Kellner 2014); 0,89 (Kellner 2014)
LC elution order/characteristicsbetween U and G (Kellner 2014, Kellner 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -
Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry. Su D, Chan CT, Gu C, Lim KS, Chionh YH, McBee ME, Russell BS, Babu IR, Begley TJ, Dedon PC... Nat Protoc [details] 24625781 -
Absolute and relative quantification of RNA modifications via biosynthetic isotopomers. Kellner S, Ochel A, Thuring K, Spenkuch F, Neumann J, Sharma S, Entian KD, Schneider D, Helm M... Nucleic Acids Res [details] 25129236 -

Download [.mol file for m7G]

Comments

m7G has a positive charge at neutral pH.

Chemical groups contained

methyl groupmethyl at aromatic N

Reactions producing 7-methylguanosine

Name
G:m7G

Reactions starting from 7-methylguanosine

Name
m7G:m2,7G

Occurrence in PDB structures

PDB-ID chain residue
1ag5 A X /6
1av6 MGT /401
1c0a B G7M /646
1c2w B 7MG /745
1c2w B 7MG /2069
1c2w B 7MG /2251
1efw C G7M /46
1efw D G7M /46
1ehz A 7MG /46
1evv A 7MG /46
1fcw A 7MG /46
1fcw B 7MG /46
1fcw C 7MG /46
1fcw D 7MG /46
1fcw E 7MG /46
1fir A 7MG /46
1gix B 7MG /46
1gix C 7MG /46
1gn7 GN7 /28
1h2u X 7MG /1154
1jgo B 7MG /46
1jgo C 7MG /46
1jgp B 7MG /46
1jgp C 7MG /46
1jgq B 7MG /46
1jgq C 7MG /46
1mj1 D 7MG /46
1mj1 C 7MG /46
1mk6 A X /6
1mkl A X /6
1ml5 B 7MG /46
1n1h C G7M /1424
1ob2 B 7MG /46
1ob5 B 7MG /46
1ob5 D 7MG /46
1ob5 F 7MG /46
1qf6 B G7M /46
1sz1 E 7MG /46
1sz1 F 7MG /46
1tn2 7MG /46
1tra 7MG /46
1ttt D 7MG /46
1ttt E 7MG /46
1ttt F 7MG /46
1yfg 7MG /46
2i1c y 7MG /527
2i1c z 7MG /46
486d C 7MG /46
4tna 7MG /46
4tra 7MG /46
6tna 7MG /46

Last modification of this entry: 2017-10-06 16:00:00.083070
Edited by a user: pboccaletto
Edited content: Changed mass_prot.