Modification Summary

N6-methyladenosine

[show modification pathway]
m6A

Summary

Full nameN6-methyladenosine
Short namem6A
New Nomenclature6A
RNAMods abbreviation=
HTML abbreviation=
Enzymes ErmAM (Streptococcus pneumoniae)
ErmBC (Escherichia coli)
ErmC' (Bacillus subtilis)
Ime4 (Saccharomyces cerevisiae)
METTL14 (Homo sapiens)
METTL3 (Homo sapiens)
RlmF (Escherichia coli)
RlmJ (Escherichia coli)
RsmA (Escherichia coli)
RsmA (Saccharomyces cerevisiae)
RsmA (Thermus thermophilus)
TrmM (Escherichia coli)
Found in phylogenyEubacteria, Eukaryota
Found in RNAmRNA, rRNA, snRNA, tRNA
SMILES codeC3(OC(CO)C(O)C(O)3)N2C=NC1=C2N=CN=C1N(C)

LC-MS Informations

Sum formulaC11O4N5H15
Monoisotopic mass281.112403
Average mass281.271879
HRMS mass281.111855
Normalized LC elution time *1,66 (Kellner 2014); 1,97 (Kellner 2014)
LC elution order/characteristicsafter A (Kellner 2014, Kellner 2014)
Mass transition-132 (Kellner 2014, Su 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -
Absolute and relative quantification of RNA modifications via biosynthetic isotopomers. Kellner S, Ochel A, Thuring K, Spenkuch F, Neumann J, Sharma S, Entian KD, Schneider D, Helm M... Nucleic Acids Res [details] 25129236 -

Download [.mol file for m6A]

Chemical groups contained

methyl groupmethyl at other N

Reactions producing N6-methyladenosine

Name
A:m6A

Reactions starting from N6-methyladenosine

Name
m6A:ms2m6A
m6A:m6,6A
m6A:m6Am
m6A:A
m6A:hm6A

Last modification of this entry: 2017-07-05 16:49:38.858344
Edited by a user: pboccaletto
Edited content: Changed lc_elution_time, lc_elution_comment, lc_reference and mass_trans.