Modification Summary


[show modification pathway]


Full nameN6,N6-dimethyladenosine
Short namem6,6A
New Nomenclature66A
RNAMods abbreviationΞΆ
HTML abbreviationζ
Enzymes RsmA (Escherichia coli)
RsmA (Saccharomyces cerevisiae)
RsmA (Thermus thermophilus)
Found in phylogenyEubacteria, Eukaryota
Found in RNArRNA
PubChem ID72266
SMILES codeC3(OC(CO)C(O)C(O)3)N2C=NC1=C2N=CN=C1N(C)(C)

LC-MS Informations

Sum formulaC12O4N5H17
Monoisotopic mass295.128053
Average mass295.298867
HRMS mass295.127505
Normalized LC elution time *1,86 (Kellner 2014); 2,57 (Kellner 2014)
LC elution order/characteristicsafter m6A (Kellner 2014, Kellner 2014)
Mass transition-132 (Kellner 2014, Kellner 2014, Su 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -
Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry. Su D, Chan CT, Gu C, Lim KS, Chionh YH, McBee ME, Russell BS, Babu IR, Begley TJ, Dedon PC... Nat Protoc [details] 24625781 -
Absolute and relative quantification of RNA modifications via biosynthetic isotopomers. Kellner S, Ochel A, Thuring K, Spenkuch F, Neumann J, Sharma S, Entian KD, Schneider D, Helm M... Nucleic Acids Res [details] 25129236 -

Download [.mol file for m6,6A]

Chemical groups contained

methyl groupmethyl at other N
methyl groupsecond methyl at other N

Reactions producing N6,N6-dimethyladenosine


Reactions starting from N6,N6-dimethyladenosine


Occurrence in PDB structures

PDB-ID chain residue
1wts MA6 /8
1wts MA6 /9
1wtt MA6 /8
1wtt MA6 /9
2i1c y MA6 /1518
2i1c y MA6 /1519

Last modification of this entry: 2017-07-05 16:51:24.802169
Edited by a user: pboccaletto
Edited content: Changed lc_elution_time, lc_elution_comment, lc_reference and mass_trans.