Modification Summary

2-methyladenosine

[show modification pathway]
m2A

Summary

Full name2-methyladenosine
Short namem2A
New Nomenclature2A
RNAMods abbreviation/
HTML abbreviation/
Enzymes RlmN (Escherichia coli)
Found in phylogenyEubacteria
Found in RNArRNA, tRNA
PubChem ID261450
SMILES codeC3(OC(CO)C(O)C(O)3)N2C=NC1=C2N=C(C)N=C1N

LC-MS Information

Sum formulaC11O4N5H15
Monoisotopic mass281.112403
Average mass281.271879
HRMS mass281.111855
Normalized LC elution time *1,65 (Kellner 2014); 1,91 (Kellner 2014)
LC elution order/characteristicsbetween A and m6A (Kellner 2014, Kellner 2014)
Mass transition-132 (Kellner 2014, Kellner 2014, Su 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -
Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry. Su D, Chan CT, Gu C, Lim KS, Chionh YH, McBee ME, Russell BS, Babu IR, Begley TJ, Dedon PC... Nat Protoc [details] 24625781 -
Absolute and relative quantification of RNA modifications via biosynthetic isotopomers. Kellner S, Ochel A, Thuring K, Spenkuch F, Neumann J, Sharma S, Entian KD, Schneider D, Helm M... Nucleic Acids Res [details] 25129236 -

Download [.mol file for m2A]

Chemical groups contained

methyl groupmethyl at aromatic C

Reactions producing 2-methyladenosine

Name
A:m2A

Reactions starting from 2-methyladenosine

Name
m2A:m2,8A

Occurrence in PDB structures

PDB-ID chain residue
1efw C 2MA /37
1efw D 2MA /37

Last modification of this entry: 2017-07-05 16:32:23.215900
Edited by a user: pboccaletto
Edited content: Changed lc_elution_time, lc_elution_comment, lc_reference and mass_trans.