Modification Summary

N2,N2-dimethylguanosine

[show modification pathway]
m2,2G

Summary

Full nameN2,N2-dimethylguanosine
Short namem2,2G
New Nomenclature22G
RNAMods abbreviationR
HTML abbreviationR
Enzymes Trm-G10 (Pyrococcus abyssi)
Trm1 (Saccharomyces cerevisiae)
Trm1 (Aquifex aeolicus)
Trm1 (Pyrococcus horikoshii)
Trm1 (Homo sapiens)
Found in phylogenyArchaea, Eubacteria, Eukaryota
Found in RNArRNA, tRNA
PubChem ID601633
SMILES codeC3(OC(CO)C(O)C(O)3)N2C=NC1=C2N=C(N(C)(C))NC1=O

LC-MS Information

Sum formulaC12O5N5H17
Monoisotopic mass311.122968
Average mass311.298172
HRMS mass311.122419
Normalized LC elution time *1,62 (Kellner 2014)
LC elution order/characteristicsbetween A and m6A (Kellner 2014)
Mass transition-132 (Kellner 2014, Su 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -
Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry. Su D, Chan CT, Gu C, Lim KS, Chionh YH, McBee ME, Russell BS, Babu IR, Begley TJ, Dedon PC... Nat Protoc [details] 24625781 -

Download [.mol file for m2,2G]

Chemical groups contained

methyl groupmethyl at other N
methyl groupsecond methyl at other N

Reactions producing N2,N2-dimethylguanosine

Name
m2G:m2,2G

Reactions starting from N2,N2-dimethylguanosine

Name
m2,2G:m2,2Gm

Occurrence in PDB structures

PDB-ID chain residue
1ehz A M2G /26
1evv A M2G /26
1f7u B M2G /926
1f7v B M2G /926
1fcw A M2G /26
1fcw B M2G /26
1fcw C M2G /26
1fcw D M2G /26
1fcw E M2G /26
1gix B M2G /26
1gix C M2G /26
1jgo B M2G /26
1jgo C M2G /26
1jgp B M2G /26
1jgp C M2G /26
1jgq B M2G /26
1jgq C M2G /26
1mj1 D M2G /26
1mj1 C M2G /26
1ml5 B M2G /26
1ob2 B M2G /26
1ob5 B M2G /26
1ob5 D M2G /26
1ob5 F M2G /26
1sz1 E M2G /26
1sz1 F M2G /26
1tn2 M2G /26
1tra M2G /26
1ttt D M2G /26
1ttt E M2G /26
1ttt F M2G /26
1yfg M2G /26
2i1c y M2G /966
486d A G /26
486d C M2G /26
4tna M2G /26
4tra M2G /26
6tna M2G /26

Last modification of this entry: 2017-07-05 16:20:35.035478
Edited by a user: pboccaletto
Edited content: Changed lc_elution_time, lc_elution_comment, lc_reference and mass_trans.