Modification Summary

5-hydroxyuridine

[show modification pathway]
ho5U

Summary

Full name5-hydroxyuridine
Short nameho5U
New Nomenclature50U
RNAMods abbreviation
HTML abbreviation
Enzymes
Found in phylogenyEubacteria
Found in RNA
SMILES codeC3(OC(CO)C(O)C(O)3)N1C=C(O)C(=O)NC1(=O)

LC-MS Information

Sum formulaC9O7N2H12
Monoisotopic mass260.0645
Average mass260.2036
[M+H]+261.0717
Product ions129
Normalized LC elution time * not available
LC elution order/characteristics not available

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

Download [.mol file for ho5U]

Comments

ho5U at the wobble position of bacterial tRNA has been demonstrated in null-mutation CmoB. Null mutation of CmoA in S. enterica results in the accummulation of tRNA containing mo5U34 and ho5U34.

Chemical groups contained

otherhydroxyl

Reactions producing 5-hydroxyuridine

Name
U:ho5U

Reactions starting from 5-hydroxyuridine

Name
ho5U:cmo5U
ho5U:mo5U

Publications

Title Authors Journal Details PubMed Id DOI
Isolation of 5-hydroxyuridine (iso-barbituridine) from yeast ribonucleic acids. Lis AW, Passarge WE Arch Biochem Biophys [details] 5957714 -
The wobble hypothesis revisited: uridine-5-oxyacetic acid is critical for reading of G-ending codons. Nasvall SJ, Chen P, Bjork GR RNA [details] 17942742 -

Last modification of this entry: 2017-10-06 15:53:05.885300
Edited by a user: pboccaletto
Edited content: Changed mass_prot.