Modification Summary

uridine 5-oxyacetic acid

[show modification pathway]


Full nameuridine 5-oxyacetic acid
Short namecmo5U
New Nomenclature502U
RNAMods abbreviationV
HTML abbreviationV
Enzymes CmoB (Escherichia coli)
Found in phylogenyEubacteria
Found in RNAtRNA
PubChem ID193091
SMILES codeC3(OC(CO)C(O)C(O)3)N1C=C(OCC(=O)O)C(=O)NC1(=O)

LC-MS Information

Sum formulaC11O9N2H14
Monoisotopic mass318.0699
Average mass318.2403
Product ions187/169/141
Normalized LC elution time *0,54 (Kellner 2014)
LC elution order/characteristicsbetween C and U (Kellner 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -

Download [.mol file for cmo5U]


Earlier called the V nucleoside. Base pairs with A, G and U in mRNA. cmo5U (and mcmo5U) is a modification characteristic for gram negative bacteria (E. coli, Protebacteria). Gram positive bacteria (B. subtilis, Firmicutes) have mo5U in this position.

Chemical groups contained


Reactions producing uridine 5-oxyacetic acid


Reactions starting from uridine 5-oxyacetic acid



Title Authors Journal Details PubMed Id DOI
Uridin-5-oxy acetic acid: a new minor constituent from E. coli valine transfer RNA I. Murao K, Saneyoshi M, Harada F, Nishimura S Biochem Biophys Res Commun [details] 4910247 -
The wobble hypothesis revisited: uridine-5-oxyacetic acid is critical for reading of G-ending codons. Nasvall SJ, Chen P, Bjork GR RNA [details] 17942742 -

Last modification of this entry: 2017-07-05 14:41:30.553934
Edited by a user: pboccaletto
Edited content: Changed lc_elution_time, lc_elution_comment, lc_reference, mass_trans and papers.