Modification Summary


[show modification pathway]


Full nameN4-acetylcytidine
Short nameac4C
New Nomenclature42C
RNAMods abbreviationM
HTML abbreviationM
Enzymes NAT10 (Homo sapiens)
Rra1 (Saccharomyces cerevisiae)
TmcA (Escherichia coli)
Found in phylogenyEubacteria, Eukaryota
Found in RNArRNA, tRNA
SMILES codeC3(OC(CO)C(O)C(O)3)N1C=CC(N(C(=O)C))=NC1(=O)

LC-MS Information

Sum formulaC11O6N3H15
Monoisotopic mass285.096085
Average mass285.257042
HRMS mass285.095536
Normalized LC elution time *1,35 (Kellner 2014)
LC elution order/characteristicsbetween G and A (Kellner 2014)
Mass transition-132 (Kellner 2014, Su 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -
Absolute and relative quantification of RNA modifications via biosynthetic isotopomers. Kellner S, Ochel A, Thuring K, Spenkuch F, Neumann J, Sharma S, Entian KD, Schneider D, Helm M... Nucleic Acids Res [details] 25129236 -

Download [.mol file for ac4C]

Chemical groups contained


Reactions producing N4-acetylcytidine


Reactions starting from N4-acetylcytidine


Last modification of this entry: 2017-07-05 16:13:18.950919
Edited by a user: pboccaletto
Edited content: Changed lc_elution_time, lc_elution_comment, lc_reference and mass_trans.