Modification Summary


[show modification pathway]


Full name2′-O-methyluridine
Short nameUm
New Nomenclature0U
RNAMods abbreviationJ
HTML abbreviationJ
Enzymes AviRb (Streptomyces viridochromogenes)
MRM2 (Homo sapiens)
Mrm2 (Saccharomyces cerevisiae)
Nop1 (Saccharomyces cerevisiae)
RlmE (Escherichia coli)
Spb1 (Saccharomyces cerevisiae)
Trm44 (Saccharomyces cerevisiae)
TrmJ (Escherichia coli)
TrmL (Escherichia coli)
aTrm56 (Pyrococcus horikoshii)
Found in phylogenyArchaea, Eubacteria, Eukaryota
Found in RNArRNA, snRNA, snoRNA, tRNA
SMILES codeC3(OC(CO)C(O)C(O(C))3)N1C=CC(=O)NC1(=O)

LC-MS Information

Sum formulaC10O6N2H14
Monoisotopic mass258.085186
Average mass258.231306
HRMS mass258.084637
Normalized LC elution time *1,10 (Kellner 2014); 1,09 (Kellner 2014)
LC elution order/characteristicsbetween G and A (Kellner 2014, Kellner 2014)
Mass transition-146 (Kellner 2014, Kellner 2014, Su 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -
Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry. Su D, Chan CT, Gu C, Lim KS, Chionh YH, McBee ME, Russell BS, Babu IR, Begley TJ, Dedon PC... Nat Protoc [details] 24625781 -
Absolute and relative quantification of RNA modifications via biosynthetic isotopomers. Kellner S, Ochel A, Thuring K, Spenkuch F, Neumann J, Sharma S, Entian KD, Schneider D, Helm M... Nucleic Acids Res [details] 25129236 -

Download [.mol file for Um]

Chemical groups contained

methyl groupmethyl at O2

Reactions producing 2′-O-methyluridine


Reactions starting from 2′-O-methyluridine


Last modification of this entry: 2017-07-05 15:43:11.172230
Edited by a user: pboccaletto
Edited content: Changed lc_elution_time, lc_elution_comment, lc_reference and mass_trans.