Modification Summary

2′-O-methyladenosine

[show modification pathway]
Am

Summary

Full name2′-O-methyladenosine
Short nameAm
New Nomenclature0A
RNAMods abbreviation:
HTML abbreviation:
Enzymes Nop1 (Saccharomyces cerevisiae)
Nsr (Streptomyces actuosus)
Trm13 (Saccharomyces cerevisiae)
Tsr (Streptomyces cyaneus)
Found in phylogenyEubacteria, Eukaryota
Found in RNArRNA, snRNA, snoRNA, tRNA
SMILES codeC3(OC(CO)C(O)C(O(C))3)N2C=NC1=C2N=CN=C1N

LC-MS Information

Sum formulaC11O4N5H15
Monoisotopic mass281.1124
Average mass281.2719
[M+H]+282.1195
Product ions136
Normalized LC elution time *1,59 (Kellner 2014); 1,83 (Kellner 2014)
LC elution order/characteristicsbetween A and m6A (Kellner 2014, Kellner 2014)

* normalized to guanosine (G), measured with a RP C-18 column with acetonitrile/ammonium acetate as mobile phase.

LC-MS Publications

Title Authors Journal Details PubMed Id DOI
Profiling of RNA modifications by multiplexed stable isotope labelling. Kellner S, Neumann J, Rosenkranz D, Lebedeva S, Ketting RF, Zischler H, Schneider D, Helm M. Chem Commun (Camb). [details] 24567952 -
Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry. Su D, Chan CT, Gu C, Lim KS, Chionh YH, McBee ME, Russell BS, Babu IR, Begley TJ, Dedon PC... Nat Protoc [details] 24625781 -
Absolute and relative quantification of RNA modifications via biosynthetic isotopomers. Kellner S, Ochel A, Thuring K, Spenkuch F, Neumann J, Sharma S, Entian KD, Schneider D, Helm M... Nucleic Acids Res [details] 25129236 -

Download [.mol file for Am]

Chemical groups contained

methyl groupmethyl at O2

Reactions producing 2′-O-methyladenosine

Name
A:Am

Reactions starting from 2′-O-methyladenosine

Name
Am:m6Am
Am:Im
Am:m1Am

Occurrence in PDB structures

PDB-ID chain residue
1x9c A A2M /5
1zfr A A2M /5
1zft A A2M /5
2d2k A A2M /5
2d2l A A2M /5
2fij A A2M /5
2fij B A2M /15
2fil A A2M /105
2fil B A2M /205
2fil C A2M /305
2fil D A2M /405

Last modification of this entry: 2017-10-06 19:46:37.329127
Edited by a user: pboccaletto
Edited content: Changed product_ions.